SKU: 38416424942

Mouse TL ELISA Kit

$228.57 $253.97
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Description

Mouse TL ELISA KitProduct Specification Sensitivity 9. 375ng ml Species Reactivity Mouse Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin labeled anti TC antibody. Free components are washed away. HRP streptavidin (SABC) is

Product Specification

Sensitivity 9.375ng/ml
Species Reactivity Mouse
Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre-coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin-labeled anti-TC antibody. Free components are washed away. HRP-streptavidin (SABC) is added, and biotin specifically binds to streptavidin to form an immune complex. The unbound conjugate was washed off and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP) and turned yellow after the reaction stop solution was added. The OD value was measured at 450nm with a microplate reader. Using curve equation software, the OD450 value of the sample was compared to the standard curve to determine the concentration of TC in the sample. There is an inverse proportion between the concentration of the target substance and the OD450 value.
Synonym Mouse TC (Total cholesterol) ELISA Kit
Detection Type Competition Law
Composition
Name 9 6 T  match   set Save conditions after opening
ELISA Enzyme plate 8 Hole ×12 Strip Place the unused holes in a zippered aluminum foil bag and add desiccant and store sealed. Available at 2-8°C Save 1 Months; In -20°C Save 6 Months.
Lyophilized Standard 2 branch
Place the unused standard in the desiccant pack. Available at 2-8°C Save 1 Months; In -20°C Save 6 Months.
Concentrated biotin - Antibody (lyophilized)
1 branch
concentrate HRP- Streptavidin 100X
120uL
2-8°C( Protect from light )
TMB Chromogenic substrate
 1 Bottle 10ml
Purified water
 200ul
 2-8°C
 
 
Sample dilution
 1 Bottle 20ml
Antibody dilution
 1 Bottle 10ml
 SABC Diluent
 1 Bottle 10ml
Reaction stop solution
 1 Bottle 10ml
Concentrated wash 25X
 1 Bottle 30ml
Coating
5 Zhang
 
 
Instructions
1 Share
Note: The volume of liquid reagent supplied in the reagent bottle is slightly larger than the label indicates. Please use a pipette to measure accurately and dilute accordingly.
General Notes 1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure.
2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture).
3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation.
4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds.
5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure.
6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation.
7. Do not reuse disposable tips to avoid cross-contamination.
8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature.
9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.
Instructions Serum, plasma, cell culture supernatant, cell lysate and other fluid samples
Storage Temp. Unopened kit, stored at 2-8 °C, shelf life for one year.
Test Range 15.625-1000ng/ml
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Exchange/Return Notes
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SKU: 38416424942

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Carnegie, US
★★★★★ 5
Completes my festival looks and doesn’t fall off
Pattern Name: classic
Definitely stayed stuck on my face even after I partied a little too hard (ifkwim), and I sweat a lot too! If it does peel off at the edges a little, eyelash glue is a best solution!
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Reviewed in the United States on July 18, 2023
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Love the variety
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Perfect for standing out at festivals!
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Reviewed in the United States on April 4, 2025
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ltrepp
Phoenix, US
★★★★★ 5
Adorable
Pattern Name: classic
These were perfect for the girls night out, our last night of camp. Everyone wanted to know where we got them. Not itchy or reactive to sensitive skin.
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Reviewed in the United States on July 7, 2024
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Amazon Customer
Omaha, US
★★★★★ 5
My picky dog loves them.
Size: 3.5 Ounce (Pack of 2), Style: Chicken Chips, Size: 3.5 Ounce (Pack of 2), Style: Chicken Chips
I try to give my dog, whom I love so much, the healthies food and treats that i can. She is so very picky and it's difficult to find things she likes. She loves these treats and expectes one every morning. She litterally waites by the pantry door for one. The are healthy Human grate chicken chips. Makes me feel better about giving her a treat. I would Highly recommend.
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Reviewed in the United States on May 13, 2026
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E J W
Lexington, US
★★★★★ 5
Treats
Size: 3.5 Ounce (Pack of 1), Style: Chicken Chips
Puppy likes them
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Reviewed in the United States on April 21, 2026

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