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Description
Human TREM2 ELISA KitProduct Specification protein TREM2 Usage 1. Self brought consumables: 1 , plate reader (capable of measuring 450 nm Absorbance at) 2 , multi channel pipette or automatic plate washer 3 , incubator, cryogenic centrifuge 4 Adjustable pipette gun and gun tip 5 Deionized water 2. Reagent preparation: Standard Diluent Ready to use; Before use, equilibrate to room temperature; 2 8 Save. Standard Diluent Used to dilute standard. 1Assay Buffer Before use,
Product Specification
| protein | TREM2 | ||||||||||||||||||||||||||||||||||||||||
| Usage |
1. Self-brought consumables: 1 , plate reader (capable of measuring 450 nm Absorbance at) 2 , multi-channel pipette or automatic plate washer 3 , incubator, cryogenic centrifuge 4 Adjustable pipette gun and gun tip 5 Deionized water 2. Reagent preparation: Standard Diluent ： Ready-to-use; Before use, equilibrate to room temperature; 2-8℃ Save. Standard Diluent Used to dilute standard. 1×Assay Buffer ： Before use, equilibrate to room temperature and press with deionized water 1 ： 10 Proportional dilution of Assay Buffer (10×) get 1×Assay Buffer , mix gently to avoid foaming. 2-8℃ Preservation, this solution can be stable 30 Heaven. 1×Assay Buffer For diluting serum and plasma samples, HRP-conjugated Human TREM2 Detect Antibody (100×) 。 Human TREM2 Standard ： The label superscript added to the standard focuses on suspension volume reconstitution in deionized water Human TREM2 Standard get 20 ng/mL Standards. Before performing dilution, hold the standard at least 15 min And gently shake, after reconstitution Standard Available at -20℃ Save 1 Months, redissolved and used 1 Discard after times. 1×HRP-conjugated Human TREM2 Detect Antibody ： Mix thoroughly before dilution. Depending on the amount required for standard and sample, use in clean plastic tubes 1×Assay Buffer Right HRP-conjugated Human TREM2 Detect Antibody (100×) Conduct 1:100 Dilution. 1×HRP-conjugated Human TREM2 Detect Antibody Should be after dilution 30 min Internal use. HRP Substrate (TMB) ： Ready-to-use; Before use, equilibrate to room temperature; 2-8℃ Store in the dark from light. Stop Solution ： Ready-to-use; Before use, equilibrate to room temperature; 2-8℃ Save. 1×Wash Buffer: Equilibrate to room temperature before use, Wash Buffer (20×) Performed with deionized water 1:20 Dilution to obtain 1×Wash Buffer 。 Mix gently to avoid blistering, store at room temperature, this solution is stable 30 Heaven. Standard curve settings: As shown in the table below, use Standard Diluent Will 20 ng/mL The standard was diluted to 10 、 5 、 2.5 、 1.25 、 0.63 、 0.31 、 0.16 And 0 ng/mL Of Human TREM2 Standards.
Note: Each experiment requires a new formulation of standards. 3. Sample preparation: 1 Cell culture supernatant: centrifuge to remove particles, immediately measure or dispense -20℃ Store and avoid repeated freezing and thawing. 2 Serum: Use a serum separation tube and allow the sample to coagulate at room temperature 30 min , then 1,000 g Centrifugation 15 min 。 Take the upper layer serum for immediate determination or dispensing -20℃ Store and avoid repeated freezing and thawing. 3 Plasma: use EDTA , heparin or citrate as an anticoagulant. After collection 30 min Inner 1,000 g Centrifugation 15 min 。 Immediate determination or dispensing -20℃ Store and avoid repeated freezing and thawing. CAUTION: Do not use specimens with severe hemolysis or lipidemia. If the sample is to be in 24 h Used within, you can store it in 2 To 8℃ , avoid repeated freezing and thawing. Frozen samples should be slowly warmed to room temperature and gently mixed prior to determination. 4. Experimental steps: 1 Remove the excess slats from the plate frame, place them back in the aluminum foil bag containing the desiccant pack, and reseal them. 2 The liquid in each well was removed and washed. Add per well using a multi-channel pipette or automatic washer 300 μL 1×Wash buffer Let stand and soak 30 s , performing washing. Complete removal of the liquid in each step is essential to obtain good performance. After the last wash, the plate was inverted and blotted dry with a clean paper towel to remove any remaining 1×Wash Buffer 。 Use the plate immediately after the plate wash is completed and do not allow the plate to dry. 3 , Add standard: add standard well 100 μL Diluted standards. 4 Add sample: serum / Plasma: sample well addition 80 μL 1×Assay Buffer And 20 μL Pre-diluted samples; Cell culture supernatant: sample well addition 100 μL Cell culture supernatant (See sample preparation for details). 5 , add detection resistance, add each well 50 μL 1×HRP-conjugated Human TREM2 Detect Antibody , guarantee step 3 、 4 、 5 Add samples continuously without interruption. The sample loading process is 15 min Within completion. Plate was coated and incubated at room temperature 2 h 。 6 Repeat steps 2 Washing in 6 Times. 7 , adding per well 100 μL HRP Substrate (TMB) 。 The plate was coated and incubated at room temperature protected from light 5-30 min 。 8 , adding per well 100 μL Stop Solution ， Stop Solution Should be in accordance with the TMB The same order is added to the plate. The color in the hole should change from blue to yellow. If the color in the hole is green or the color changes unevenly, tap the plate lightly to ensure thorough mixing. 9 , in 30 min Within, measure each well 450 nm The absorbance value at. Note: 5-30 min The color development time is within the empirical range. For each specific experiment, the approximate color development time can be determined according to the following conditions. （ 1 ) Visual observation: standard song S5 The holes are light blue, Blank When the hole has no obvious blue color, it can be terminated; （ 2 ) Instrument judgment: 630 nm At left and right wavelengths, standard curve S1 Porous OD Value reaches 0.5 - 0.7 、 S5 Porous OD Value reaches 0.05 - 0.08 、 Blank Porous OD Value less than 0.05 It can be terminated. 5. Result calculation: 1 Calculate the multiwell average of each standard and sample OD Value, and subtract the zero concentration ( Std.8 ) average OD Value. 2 , Drawing of standard curve: take the standard concentration as x Axis, the average absorbance of each standard is y Axis, plot the standard curve. You can use mapping software to create standard curves. Note: If the sample is diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 6. Results display: Typical standard curve ( R2≥0.99 ） Figure1. 96 Well plate analytical Human TREM2 Standard curve. The data and curves are for reference only, and experimenters need to establish standard curves according to their own experiments. |
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| Sensitivity | 0.16 ng/mL | ||||||||||||||||||||||||||||||||||||||||
| Species Reactivity | Human | ||||||||||||||||||||||||||||||||||||||||
| Source | Human | ||||||||||||||||||||||||||||||||||||||||
| Synonym | Human TREM-2 ELISA Kit | ||||||||||||||||||||||||||||||||||||||||
| Detection Type | Sandwich method | ||||||||||||||||||||||||||||||||||||||||
| Description | Myeloid cell trigger receptor 2 (TREM2) is a single Ig domain receptor that is expressed in macrophages and dendritic cells, but not in granulocytes or monocytes. It is most abundantly expressed in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, while microglia in the central nervous system are the most dominant cell type producing TREM2. TREM2 plays a role in chronic inflammation and can stimulate the production of constituent rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP to activate the immune response of macrophages and dendritic cells. It is also associated with the adaptor protein DAP12 to transmit activation signals, which play a role in innate and adaptive immune responses. TREM2 Signaling is also an important way to promote colonic wound healing. TREM2 mutations increase the risk of neurodegenerative diseases such as Alzheimer's disease, amyotrophic lateral sclerosis, and Parkinson's disease. The Human Myeloid Cell Trigger Receptor 2 ELISA Quantification Kit employs a double antibody sandwich method to quantify human myeloid cell trigger receptor 2 in a sample. The plate is coated with an antibody specific to human myeloid cell trigger receptor 2, and the sample or standard and the specific detection antibody labeled with HRP are added to the wells of the plate. The human myeloid cell trigger receptor 2 binds to the solid phase antibody and the detection antibody coated on the plate. After washing, a chromogenic substrate (TMB) was added, and the TMB became blue under HRP catalysis and turned yellow after addition of the stop solution. The OD value was measured with a microplate reader at 450 nm wavelength, and the concentration of human myeloid cell trigger receptor 2 was directly proportional to the OD450 value. |
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| Composition |
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| General Notes | 1. If Standard Diluent and Assay Buffer (10 ×) turn yellow in color and have a small amount of precipitation, it is because the reagent contains serum. Just centrifuge to remove the precipitation, which will not affect normal use. 2. Do not mix components with different batch numbers and different manufacturers; Failure to do so may lead to abnormal results. 3. Change the tip frequently to avoid cross-contamination between the components. 4. To ensure accurate results, the plate must be properly adhered during the incubation step. 5. Stop Solution is corrosive to a certain extent, so please take protective measures when operating. |
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| Instructions | Serum (plasma), cell culture supernatant | ||||||||||||||||||||||||||||||||||||||||
| Storage Temp. | 2-8 ℃, protected from light, valid for 24 months. | ||||||||||||||||||||||||||||||||||||||||
| Test Range | 0.16 ng/mL-10 ng/mL |
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4.6 ★★★★★
Based on 1788 reviews
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Product Reviews
★★★★★ 5
4 Paws up! My dogs LOVE them
Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large, Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large
I have 2 large dogs who LOVE THESE.
A Great Pyrenees mix who’s a skinny 80lbs and a lab/terrier mix who’s a chunky 50lbs. They both love bones and can REALLY chew through them quickly so I’m really picky about what I buy them.
Here is what is important to me and how I rate and choose bones. These are my comments on this bone set specifically:
Do they break/splinter?
-They don’t splinter, I have seen smaller pieces that have broken off and when the bone gets down to the end (but this is normal with bones) at which point those pieces can be thrown away. I have never had an issue with them choking, being too small or hurting my dogs though and they are aggressive chewers so I could see this happening with some dogs and never happening with others. 4/5 stars for this.
Do they smell/leave stains on the carpet?
-Nope! No odor and they actually leave my dogs breath smelling better and their teeth cleaner! 5/5 stars
Does it upset my dog’s stomach? (My bigger guy can’t tolerate a LOT of things, he vomits often and also gets diarrhea if he eats the “wrong” thing so I have to be very cautious.)
-NOT at all! No upset tummies. This is always very, very important to me. Make sure you have lots of water nearby for your pups though, these will make them very thirsty. 5/5 stars
Do they hurt my dogs tongue, mouth or teeth?
-Nope, no issues with this. They’re able to gnaw into these aggressively without hurting themselves. 5/5 stars
Are they gone in 2 seconds?
-No! I’m always worried the “stuffed” bones will be left in the dust after the stuffing is all gone but my dogs will still keep chewing on these after the stuffing is gone. They still enjoy the bones and they last for quite some time, weeks at minimum. I can also stick some peanut butter or other treats back in the middle after they get the original stuffing all out of them to “reuse” them if it seems like they’re getting a little bored with them. 5/5 stars
Do my dogs ACTUALLY LIKE THEM?
-They love them. 5/5 stars
Price?
-Great and totally affordable. $20 for 3 of them.. so less than $7/bone. This is comparable to a bone from the pet store and these last a lot longer than something you would find there in the “fresh” grocery-store-like-section of the pet stores.
I’m an avid review reader, especially when it comes to items for my fur babies because I want the best for them. I truly love these bones and will keep buying them.
I will continue to buy these!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 26, 2019
★★★★★ 4
⭐️⭐️⭐️⭐️ Finally Found a Long-Lasting Bone That Actually Keeps My Dog Busy
Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large
I’ve tried a lot of filled bones and chew treats over the years, and most fall into two categories: gone in 5 minutes, or upset stomach later. These filled beef shank bones hit the sweet spot.
The bone itself is thick, dense, and real, not a thin, brittle shell. My medium-large dog is an aggressive chewer, and this keeps him occupied for a solid 30–45 minutes working on the filling, then even longer gnawing on the bone afterward.
The filling smells fresh and appetizing (not rancid or overly greasy), and my dog goes crazy for it. We’ve tried the cheese-style and peanut butter–style fillings, and both were a big hit. No crumbly mess, no weird residue, and no digestive issues so far.
What I really appreciate is that these feel safer than many cheaper alternatives. The edges don’t splinter, and the bone wears down gradually instead of cracking.
Why I’ll keep buying these:
• Long-lasting even for power chewers
• Real beef shank bone, not pressed junk
• Filling dogs actually care about
• Helps with boredom and anxiety
• Good value compared to pet store brands
Minor cons:
• Can get a little smelly after heavy chewing
• I supervise and take it away when it gets small (as with any bone)
If you’re tired of wasting money on chews that disappear in minutes, these are a fantastic upgrade. My dog is obsessed, and I finally feel good about what I’m giving him.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 5, 2026
★★★★★ 5
Best bones out there
Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large
These are the best bones I’ve ever bought. I have a lab puppy and as you can imagine most bones don’t last long around here, but this one keeps him entertained for days.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 4, 2026
★★★★★ 5
Happy doggy dancing my ensue
Flavor Name: Variety Packs, Size: 1.26 Pound (Pack of 1), Set name: Small
I haven’t tried them, but the dogs seem to like them. They do that tippy tappy happy dance when these come out.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 26, 2026
★★★★★ 3
Sturdy
Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large, Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large
Ok so I'm very happy that my standard poodle seemed to really enjoy this product. She was lacking and biting it for hours and days. That said she has still yet to make a dent in the bone. It seems to be too hard and too thick for her. I was surprised how heavy it was! It is large enough it is completely safe to leave her in the other room with. No strange odor or transfer of color. I'd say for the cost it isn't worth it as she is only getting filling and not able to eat the bone for some reason. Then again she is entertained for hours..so maybe so. Doesn't seem to bother her sensitive tummies either.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 7, 2026
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